Artificial inoculation

人工處理植入催化劑

Artificial inoculation technique already been developed and is found to be most effective and reliable method for enhancement of agar production. Works on commercialization are in progress and expected to make available for general use in the field.

The Agarwood created by artificial inducement is called Cultivated Agarwood or Induced Agarwood. Artificial inducement technique (or Agarwood inducement) consists of two actions: to wound the tree and to pour stimulative agent into this injury, this stimulative agent hereinafter referred to as inoculant. Quantity and quality of cultivated agarwood surrounding the injury depends on the composition of inoculant and the method of transfusing inoculant into the tree.

Nowadays, there are about several tens of inoculants whose composition is kept secret. But in general the main components of inoculant are:

  • Some fungi and microfungi, for example: Fusarium oxysporum Schlecht., Cladosporium spp, Cercosporella spp, Aspergillus Phoenicis (Corda) Thom & Currie, Cytosphaera Manganiferae Died, Melanotus flavolivens (BerPlant hormonesk. & M.A. Curtis) Singer, Penicillium Citrinum Thom, Chaetonium globosum Kunze, Phaeoacremonium parasitica,…in which Fusarium, Cladosporium and Cercosporella are three of 17 fungi that were found in 9 natural agarwood samples collected from the forest in Thailand, Fusarium and Chaetonium are two fungi found in natural agarwood pieces collected from India.
  • Some phytochemicals, phytohormones namely: salicylic acid, ethephon, methyl jasmonate, chitosan, sodium bisulfite,..
  • Some bacteria, yeast extracts, plant nutrient media and plant regulators

Two main methods Agarwoodof transfusing inoculant into Aquilaria tree:

Dispersing wounding by mechanical injury: to drill several tens (20-100) of hole into which the inoculant is poured. In this case induced agarwood is formed around these spot injury sites. The resulting agarwood from the localized formation is usually in the form of thicker wood chips.

Internal wounding: to transfuse inoculant directly into tAgarwoodhe xylem vessels of tree by using transfusion set, in this case only few holes are needed. If the internal wounding technique to be applied then induced agarwood is formed in whole tree along the xylem, from root/base up to the top of the trunk in a systemic manner, but relatively thinner formation of resinous wood.

Lately, inoculant operators have adopted to Dispersing wounding, leaving the Internal wounding for few reasons after gaining numerous field experiences … wood rotting is the main drawback.

gaharu

wood rotting by internal wounding

While internal wounding is preferred by certain people, it can be further improved with “Pressurized method”. The air pressure can be portably pumped range 0.5 ~ 1.5 bar, hence minimize local concentration which resulting in tissue rotten. For cost effective, unit can be connected to multiple points, and reusable in next application. This invention is simple, inoculation process completed at faster time, no ingress of rain water even the injection holes are not sealed. Click “Mini pump“.

Pressurized method

Dispersing wounding, mechanically wounded with nails, traditional way in Bangladesh

Dispersing wounding, tree drilling and chemical injection.DSC_4217-Edits800

This effort involves drilling of trees and keeping the wound open by placing a small piece of plastic pipe in those holes followed by a chemical injection to stimulate tree defense mechanism that produces resin. The first project of this effort was initiated in Vietnam under a supervision from Prof. Robert Blanchette, a wood pathologist from the University of Minnesota who together with local farmers and Buddhist monks. They developed experimental plots to stimulate the production of agarwood and after years of trials, this treatment yielded agarwood. Dr. Blanchette stated that this artificial induction could yield agarwood ten times faster than natural formation (WWW page: http://forestpathology.coafes.umn.edu). This finding has gained appreciation and has been considered as one of the most successful finding (Persoon, 2007). This treatment causes tree to respond in two defense mechanisms, physical and chemical. The first is phloem cells form callus and the second, should callus formation is prevented, the tree produces resin.

Dispersing wounding with 10~20ml liquid inoculant, some inoculant service providers utilize upto 100ml per treated hole.

Dispersing wounding 1Dispersing wounding 2Dispersing wounding 3Dispersing wounding 4

Dispersing wounding, inoculant-soaked bamboo stick, Thailand
CIMG0467

Dispersing wounding, inoculation of fungal inoculum6158739645_8725dbb2b6_b

The formation of agarwood is a result of plant defense mechanism towards fungal attacks by producing resinous compounds as secondary metabolite. In their natural habitat, the process of resin accumulation as the result of tree-pathogen battle may take many years and the longer the process takes place the more expensive and highly valuable the resin is. Many scientists have been passionately trying to understand the cascade process of this tree-fungi interaction in producing gaharu. Isolation of various fungi from infected trees have been widely reported.

Surface Inducement Technique ( Agar-SIT )… inoculant is designed to brush on the tree trunk. This technique allows easy agarwood collection, a considerably high yield and good resinous of agarwood could be harvested, along with the sustainable agarwood formation from the same tree. Specifically, this technique is aimed for oil production. Click “Agar-SIT-105” for detail …

trunk surface inoculantTrunk stree

Dispersing wounding, non-destrustive harvest, by Pheeraphan Thailand

Thai dispersing 1

gaharu

agarwoodagarwood

Thai dispersing 2Thai dispersing 3

Thai dispersing 4

The infected tissue is stripped by chisel

Internal wounding, Single unit, 300ml inoculant per tree,  by PENGHARUM.

Internal wounding, Multiple units, by Gaharu Biotech,Pahang.

Picture2

Gravitically flow.

wounding

Application of Chemjet, pressure exerted 0.75bar. Test plot to verify strength of inoculant

Inoculant on SinensisClick Here

Artificial fire method, China人工火烧法It is to burn the live agarwood tree with a large fire, forcing the agarwood tree to be injured. It looks very cruel, but it does not let the agarwood tree die, so the agarwood tree will mobilize the resin secreted by the immune system to heal the fire, and eventually form agarwood. Select the older tree, peel off the bark from the tree, leave the top leaves, and then use  gas torch to burn the whole tree and burn it black. This method is derived from the natural fire or the process of lightning strikes. The agarwood produced in this way is mostly thin, it is used a lot to produce incense.

In China, newer species of Kynam has been successfully grafted, this species requires mechanical stress without any injection of liquid inoculant to produce resinous agarwood. Application of soldering device is sufficient for the job. However, the seedlings demand extremely premium price for the moment, click “Kynam

kynam23.jpgkynam24

 

 

 

 

 

 

 

Planting money (Agarwood) trees doesn’t come without its challenges. One of these challenges is the inoculation. The Aquilaria tree has to be induced with a infection to induce production of agarwood (the resin), otherwise the tree would be quite worthless. Not all Aquilaria trees produce agarwood, they have to be infected by a certain kind of stress before they start “fighting back” with resin production to counter the infection. And even so, there is no set volume in the resin production (meaning each tree will produce its own amount).

Newbies blinded by the agarwood get-rich-quick scheme are often scammed through these so-called high quality inoculant which “guarantees” high rates of return. Till today, techniques are still being improved to artificially induce optimum agarwood production. There are “many roads that lead to Rome”, with trial and error being part of the journey but there are no guarantees.

The inoculation cost for a 5-8 years old tree in Malaysia ranges 30 ~ 50USD /tree inclusive labour (Time of writing … Dec 2018). Being a layman grower, this can be an astonishing figure if you have 1000 trees to inoculate.

Try this idea …. success

This product is available in local market (Malaysia), but it is sold under different trade name, it is mainly used to treat Durian disease, such as Canker. Phosphite is used as a biodegradable fungicide to protect plants against Phytophthora dieback. Phosphite (PO3) molecules, which move systemically via the phloem and xylem to the leaves and roots. This means that an entire plant receives many benefits of the phosphite molecule. With phosphite, a plant’s natural defense mechanisms are heightened … Phosphite encourages the nucleus of a plant cell to produce defensive molecules such as phytoalexin, which defense the tree from attacks by external intruders … you may try this at higher dosage to blend your inoculant recipe.

Do not judge the book by its cover…. whether the inoculant is purchased from 3rd party or home-made, judging on the outlook of cultivated wood alone is insufficient without performing the oil analysis. To analysis the content of VOCs in “cultivated agarwood” we have to extract its essential oil and make GC-MS analysis on this essential oil for seeking specific compounds of natural agarwood. Click “Oudh quality” for detail … and NEVER proceed with mass inoculation without understanding the content of GC-MS report.

Whatever informations posted here is FREE to share, nevertheless if you required Private sitefor inoculant guidance, you may whatsapp me @

WhatsApp  : Tee … 00 – 6 – 012 – 323 1161

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140 Responses to Artificial inoculation

  1. hslglt says:

    Trying whole tree inoculation with success. Thanks gaharujinkou for your valuable guide & lively information that you have provided. Salute…

  2. Ranjit Timung says:

    Good idea .pls write about the details process and name of chemical used.

  3. Ranjit Timung says:

    Wow .pls write about the details process and chemical use

  4. Jeffery Allen Racule says:

    I am from Fiji and have planted arga for the 1st time, please need your advise on the process

  5. Dr. Md. Daulat Hussain says:

    Pl. give us the address of fungal inoculam SELLERS for inoculation of agar trees. Many agar tree growers will be benefited. Large number of agar tree farmers interested to buy FUNGAL INOCULAM FOR AGAR TREE inoculation..

  6. Khandaker Imran Hossain Topu says:

    Dear sir, I have a very small project of agar woods in Bangladesh for last 16 years which we planted. Now it’s taller like 3.5/ 4 meters almost & I am sure it is perfect time to inject fungal inoculam. Please give me the address of fungal inoculam SELLERS for inoculation of agar trees.

  7. Karma Bhutan says:

    Sir,
    I have 40-50 agarwood trees of age 25-30years…from were can i buy the fungal inoculation. Kindly help me please.
    Thank you.

  8. PARAMA NANDA GOGOI says:

    Sir, I am from ASSAM , India,Pl. give me a the address of fungal inoculam SELLERS for inoculation of agar trees.I want to buy its.

  9. Camillus Midire says:

    Pls send me details of your innoculation process.

  10. PARAMA GOGOI says:

    Respected
    Sir,Once I mail you, regarding Agar-wood inoculation ; Plz tell me the saler of inoculation.

    Regards
    PARAMA GOGOI
    ASSAM GUWAHATI.
    INDIA

  11. tshinkou says:

    Whatsapp me @ 00 – 6 – 012-323 1161

  12. Philip Yaditing says:

    I would like to buy the resin for inoculation of agarwood. What is the number to contact.

  13. William John says:

    Dear Sir,

    Iam William John from Western Province of Papua New Guinea. I have thousands of natural agarwood in my village which are 50 to 100 meters tall and they come in different sizes.
    Please can you provide me contact address of Agar-wit inducer suppliers so that I can purchase some to induce my agarwood…

    My gmail address is wxsanjohn@gmail.com

    Your response is highly appreciated.

    William John.

  14. Baby paul says:

    Please let me know the price of this

  15. Hello sir, I’m from Bhutan and I have around 100 plus agar wood trees which is around 40 to 50 plus years old. Please help me to get inoculation kit.

  16. Van ut says:

    Bài viết rất hửu ích. Cám ơn tác giả rất nhiều!

  17. Peter Mundrau says:

    Please I rely need the inoculation chemical..

  18. Kalyan dutta says:

    I need artificial fungal.. Where’ll get.. Plzz send me contract details

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