Artificial inoculation


Artificial inoculation technique already been developed and is found to be most effective and reliable method for enhancement of agar production. Works on commercialization are in progress and expected to make available for general use in the field.

Mechanically wounded with nails.

Tree drilling and chemical injection.DSC_4217-Edits800

This effort involves drilling of trees and keeping the wound open by placing a small piece of plastic pipe in those holes followed by a chemical injection to stimulate tree defense mechanism that produces resin. The first project of this effort was initiated in Vietnam under a supervision from Prof. Robert Blanchette, a wood pathologist from the University of Minnesota who together with local farmers and Buddhist monks. They developed experimental plots to stimulate the production of agarwood and after years of trials, this treatment yielded agarwood. Dr. Blanchette stated that this artificial induction could yield agarwood ten times faster than natural formation (WWW page: This finding has gained appreciation and has been considered as one of the most successful finding (Persoon, 2007). This treatment causes tree to respond in two defense mechanisms, physical and chemical. The first is phloem cells form callus and the second, should callus formation is prevented, the tree produces resin.

Insert with inoculant-soaked bamboo stick to stress tree, Thailand

Tree drilling and inoculation of fungal inoculum6158739645_8725dbb2b6_b

The formation of agarwood is a result of plant defense mechanism towards fungal attacks by producing resinous compounds as secondary metabolite. In their natural habitat, the process of resin accumulation as the result of tree-pathogen battle may take many years and the longer the process takes place the more expensive and highly valuable the resin is. Many scientists have been passionately trying to understand the cascade process of this tree-fungi interaction in producing gaharu. Isolation of various fungi from infected trees have been widely reported.

ICU method — Single unit, 300ml inoculant per tree,  by PENGHARUM.

ICU method — Multiple units, by Gaharu Biotech,Pahang.



92 Responses to Artificial inoculation

  1. ripal chakma says:

    dear sir,
    Your post is really helpfull and very informative.
    from bangladesh, how i can get ICU inoculant and how much the price?

    • tshinkou says:

      I suggest to use “Bamboo stick”, many types of inoculant available in the market, you really have to do your own survey. I have seen Bamboo stick result, it is proven.

      • Ripal Chakma says:

        dear tshinkou, thanks a lot for giving me your valuable advice. but i m from khagra chari hill district, which is very remote area of bangladesh. i really don’t have any idea of “Bamboo stick”.
        can i make ” Bamboo stick” by using local bamboo? if i can’t,then how i can get this n
        how much?
        thanks again.

  2. tshinkou says:

    These Bamboo sticks have been soak with unique inoculant a day or 2 prior to delivery by manufacturer. ICU method will cause rotten in the tissue, you still get the gaharu but harvest is reduced, The shape of gaharu is hollow in the middle. Pls click “” for Bamboo stick detail.

  3. NUr Sakinah Ismail says:

    how long does it takes to see the result for each technique?

  4. tshinkou says:

    Most of the techniques recommend a minimum 2 years for better result, the idea is, longer waiting time better the quality.

  5. Kenneth Wu says:

    Hi Tshinkou,

    Very informative website but I can’t find the name of Bamboo Stick manufacturer in the products catagory. Please let me know the name and the address of the manufacturer. Thanks.


  6. tshinkou says:

    The invention of such product is definately not from the big corporate. Many Agarwood growers claim thier products are the best, seriously i have tried few inoculants, and awaiting for the result.
    However i have seen the Bamboo stick “Cultivated Agarwood” in mass production, that why i am sharing my thought here that Bamboo stick might be one of the priority in the consideration list.
    Try a pack or 2, result will tell which is the best, happy surfing.

  7. Francis says:

    Thanks, your website is very informative. May i know the supplier of Bamboo Stick inducer in Malaysia.

    • Johny thomas says:

      Dear Sir,
      I am interested in bamboo Stick inducer.
      I have only one tree of 13 years old. Recently only I came to know about the value of it.
      How many sticks I have to use?
      Can you reply with details

  8. CJ says:

    can i know what are the inoculant use for the agarwood

  9. tshinkou says:

    Dozen of Inoculant available in the market, they are mainly home-made products. Unable to tell what are they. You can invent one of them and name it with your commercial name. Seriously, everyone claim their products are the best.

  10. Htun Wai says:

    Thanks, your website is very informative.

    • tshinkou says:

      Many veteran players refrain themselves from sharing their knowledge and hard-earned experiences, and hardly you will bounce to them in the net.

    • Htun Wai says:

      I would like to know agarwood inoculation with EM 1 method is a best one.

      • tshinkou says:

        Effective Microorganisms or EM is a mixed culture of beneficial microorganisms that can be applied as an inoculant to increase the microbial diversity of soils. This in turn, can improve soil quality and health, which enhances the growth, yield, and quality of crops.

        EM, my recommendation here is to promote plant growth. I don’t mean to inoculate the tree with EM, but you may try EM1 coupled with your own recipe.

  11. Golap Gogoi says:

    Hi, Very informative page, Recently I read about Agar WIT Technique from‎ . m trying this type of method in my place Assam, India and waiting for the result.

    • tshinkou says:

      Thanks for shaing. Ensure you are confident with the products chosen.

    • Sham says:

      Hi Golap,

      I’ve also read about the Agar wit technique.. Can I know, where I can purchase the Agar witt? By the way, Im from Brunei. Thanks.

    • Johny thomas says:

      Dear Golap
      I am from Kerala. I have an Agar tree of 13 years old. Can you give more details about Agar wit method?
      Where the chemicals are available?
      Can you please give your contact details?
      My 00968 99053791( in Oman)


    • Patrick Tan says:

      Hi Gogoi, I hv also read about it and wonder how is yr progress of using It. I am keen to acquire some for testing & wonder what is the cost for per tree and how much is required for each tree and for how long? I am also wondering what tree age or size would be idea for agarwit testing?

    • Nilesh Patel says:

      Hello Sir… I am Nilesh from Gujarat, India, Plz give me details about agar inoculation mathod, I will pay you, My email id is and my mobile no. is +919426962861 Thank you…..

    • kaushik says:

      Hi Golap,
      Recently came to know about agar wit, would also like to try it. Have you got the result. I am also from assam where from to get technology and price thereof.

    • kaushik says:

      Hi Golap,
      Recently came to know about agar wit, would also like to try it. Have you got the result. I am also from assam where from to get technology and price thereof.
      Mobile 9401532257

    • prasanth says:

      Hi Golapgi,
      Im from kerala ,i would like to know where we can get the fungus for doing the artificial inoculation,can you please give your contact number,+919747332551

  12. Mr. Zul says:

    Hi Mr. Tee,
    That mean inducer better than inoculant technique? is it bamboo stick can be used for any species of aquilaria?

    • tshinkou says:

      Each method has its unique Pros and cons, but inducer way is the improvement method from the inoculant after years of experience of the inventor. Yes, regardless of the species.

  13. Azwaj says:

    Thanks a lot for your very informative page.
    We have gone through your impressive page while browsing latest inoculation techniques for our agar plants which have become matured. You are, perhaps, aware of Bangladesh and we have about 60,000 agar plants. We need to start inoculation in coming monsoon. In Bangladesh, nailing is the only traditional way of doing this. Although CA kits have been applied in earlier years, it could not bring any success rather damaged most of the inoculated plants.
    Based on your experience, modern technology & all the other aspects, it would be great if we can get your kind advice and assistance regarding Agar Inoculation Techniques that can be followed by us in Bangladesh in the upcoming days.

    Would like to receive your reply at your earliest convenience.

  14. Aruna says:

    Thank you for your great information’s.It’s very helpful to me I wanna know what are the chemicals we should use for the artificial inoculation in agar wood?

  15. S.Wijesiri says:

    I am a project writer and presently writing a book beneficial rural Sri Lanka .Your approval sought to COPY the pictures in the page attention is highly appreciated.

  16. S.Wijesiri says:

    Walla gyrinops one of an agarwood species available in Sri Lanka which is under threat of extinction.Low income rural villagers involvement is very high in this process. Improvement on knowledge on agrwood , making income sources reduce this devastation.My book target these goals. Your approval sought to copy pictures available in
    Early response is highly appreciated.

  17. SHINODA says:


  18. abdullah ahmed says:

    hello mr
    tshinkou… i am from india and also a student of microbiology. as i am working on my summer project on artificial inoculation of agarwood.. so i want to talk to you in mail. please if u mail me… i wil be waiting for your swift reply sir
    thank you

  19. kyaw Myo Aung says:

    I have about 7000 trees (Supintegra ) 4 years old. May I know the effective CA kit for it. I plant in Myanmar(Burma). I would like to buy CA kit. Could me advice pls. thanks

  20. David says:


    can you please inform if you tried the Agar Wit Method and if successful where we may purchase the kits

  21. Lalremi Zote says:

    I have more than 1,00,000 agarwood trees, which are around 30 years now, some are above 50 years old. I want to inoculate them. But i don’t know where to purchase or use them. Please give me some advice. I would be very happy to get information about it.

  22. Moses Anawe says:

    Please I really need to get the ICU Inoculant so how much does it cost?

  23. Milon Chowdhury says:

    I am from Bangladesh. I need some information about nailing method such as nailing period, time required and result. Please reply as soon as possible.

  24. Johny Thomas says:

    I am interested to know more about agar wit inoculation method. Please advice the details of method.
    I am from Kerala and I have 10 year old tree.
    Please reply. My contact 00968 9905 3791 oman
    In India 094970 77555

  25. tshinkou says:

    Bamboo sticks method is an improvement version to Liquid Inoculation when the inventor founf much rotten occured to the tree which further reduced to yeild, the inventor finally adjusted the dosage to suit field application. This does not indicate that liquid inoculation is too bad to be used, it is up to each individual to judge by himself which method suits him.

  26. budi suprayitno says:

    Informatife …makes more information..

  27. liyanage says:

    In Sri Lanka now interest for cultivation of agarwood has initiated. Information related to cultivation,different varieties on seeds and plants suitable for local conditions,technologies,places available,agar oil processing,relevant machinery,all the information now included in “Agarwood Ayojana Awastha saha Wallapatta ” available at Sarasavi Bookshop,Nugegoda and Gunasena,Pettah.

  28. wasantha says:

    I’m from Sri Lankan. I like to know about this. What are the ingredients wetter? Please help me. thank you.

    • Janaka Opallage says:

      Dear Wasantha,

      Pls contact me on 0094 776029162, so I will give you details on buying Agar plants, growing them, inoculating and harvesting with a secured buy back.

      Look forward to your call

      • Dear Janaka, sorry for interference , I am from Fiji island , some one has starting planting Agarwood here. and its grow very very well because of tropical and very good mix of weather here.
        But after going through your massage to Mr Wasantha from Shirilanka.

        Makes me very interested if you can provide me with the same condition or your buy back deal .
        Please can you reply back to my email address below .
        Phn +6798668746
        Fiji .

  29. dulaldey says:

    Very very good

  30. tariqul islam says:

    dear sir.
    i am from Bangladesh. i want to know how ccan i make a best quality vaksin for agartrees.

  31. tariqul islam says:

    i shall be highly glad if you kindly give me a best quality agartrees vaksin manufacturing formula and using procedure .
    thanks .

  32. S.WIJESIRI says:

    In Sri Lanka most of the explanations and guidance on agarwood cultivation and technical aspects are available in the book”Agarwod Ayojana awastha saha Wallaptta ‘ written by S.Wijesiri,Project Consultant available at Sarasavi Bookshop -Nugegada,Godage-Maradana and Gunasena -Pettah.This has been appraised by almost all users.

  33. Subrata Sarkar says:

    Hello sir
    I m from Tripura, north east India. I have two agarwood trees. The age of both the trees are near about 20-25yrs. The size of these trees are also big with noticeable diameter. I want to use Bamboo sticks for creating agar on the trees. How many months or year will require for getting the results.I want to purchase the bamboo sticks. How many sticks will require for this operation and from which place I can find this sticks in India or how can I purchase from other country. Please reply me sir.
    Thank u

  34. galley dorjee says:

    i am from bhutan and would like to initiate the plantation of agarwood, and i got few samplings , so i would be indebted if sir could kindly provide me with an information about how to induce it and what kind of inducement we have to provide for the successful treatment and better result.. name some chemical to inoculate the plant,,i heard little about CA kit induced in the agarwood, so how effective it is? and how many years it takes to give agar ? thank you ,, hope ull get good information about it,, thank you sir,

  35. galley dorjee says:

    i am from bhutan and would like to initiate the plantation of agarwood, and i got few samplings , so i would be indebted if sir could kindly provide me with an information about how to induce it and what kind of inducement we have to provide for the successful treatment and better result.. name some chemical to inoculate the plant,,i heard little about CA kit induced in the agarwood, so how effective it is? and how many years it takes to give agar ? thank you ,, hope ull give a good information about it,, thank you sir,plex email me

    • tshinkou says:

      I am afraid i am not the one expert to comment, since cultivated wood demand is still limited as of today. If u have potential Agarwood products development, u can proceed with commercial planting. Whatsapp me at 00 – 6 – 012-323 1161.

  36. Yashawant says:

    I am from Maharashtra State in India and interested in commercial plantation of Agarwood trees. Can any one help me getting the detail of any company which can provide me good quality and authentic saplings and also provide technical support including buy back etc?

  37. George says:

    Hello Tshinkou, How are the prices iike for artificially induced agarwood vs naturally produced agarwood (end product i.e. wood chips), also in quality will the artifically induced come close to the natural one?

    • tshinkou says:

      As long as natural wood supply is avialable and buyers are able to pay….. cultivated wood may need some time to find its room in the market …most probably next 10 years, when the natural wood stock in the middle east warehouse is depleting.

  38. Abdullah Al Mamun says:

    Can anyone inform which university of department is best for higher study and research on Agar wood

  39. Paul Murimattathil Paulose says:

    Hello Tshinkou,
    I have four trees in my court yard and completed about 20 years and out of that two plants are some what grown up and bottom girth may be 30-36 inches and height more than 12 meters. Can you please suggest somebody in Kerala for the inoculation for the said trees on guarantee basis. Kindly send back a feedback as well.

    • tshinkou says:

      if someone in yr vicinity can propose something to you..

      • Paul Murimattathil Paulose says:

        One person from south kerala tod me over gsm that he will bring the mediciene and do the inoculation and he claimed also that the medicienes were imported from Malasia and can I rely up on him.

  40. tshinkou says:

    pls chat me thro whatsapp ….

  41. Gary Robert says:

    Helo sir,
    I’m from malaysia, sabah..i have 5 hectar of vacant land and was thinking to plant with agar wood..
    Your post is really helpful and adding my confindence in investing this planting.
    Can you share some information when do i can start using this method and how much does it cost for 1 tree?..
    How can i get this “bamboo stick” ?

  42. Ahmed says:

    Anyone selling pure oud oil in Bangladesh, kindly contact me via mail:

  43. Omar says:

    We have quite a few agar trees in our tea estate in Bangladesh and planing to go for inoculation. Just wanted to know can CAkits give good results?


  44. BhimRaj Thapa says:

    Do you supply CA Kit?
    How much price?

  45. BhimRaj Thapa says:

    I am in corresponding with a supplier in of CAKit Malaysia…Company name is Tanali Esteem, Selangor..Puchong. Big money at stake here. Hard saved money. Kindly help me find out if the office exists.

  46. abdulsiyad says:

    my name is abdulsiyad i am from kerala ( india ) i have some agarwoods tree and my location have morethan lakh trees i thing start the business here please help and infermation thank you please send me replay assalamualaikkum

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